British Medical Bulletin 56:588-602 (2000)
© 2000 The British Council
research-article |
In Vitro maturation of oocytes
*Department of Reproductive Science and Medicine, Imperial College School of Medicine, at Hammersmith Hospital London, UK
Department of Reproductive Science and Medicine, Imperial College School of Medicine, at St Mary's Hospital London, UK
Correspondence to Dr kate Hardy, Dept. of Reproductive Science and Medicine, Imperial College School of Medicine, Hammersmith Hospital, Du Cane Road, London W12 0NN, UK
Abstract
Only about 400 of the one million oocytes present at birth will be ovulated, while the rest will die by atresia. The ability to resuce oocytes destined to die and mature them in vitro would provide invaluable information about folliculogenesis and oocyte maturation, and could provide oocytes for infertile women. in vitro maturation (IVM) is challenging in the human because folliculogenesis is a lengthy process encompassing many complex cellular changes in the oocyte and its surrounding follicle cells. A few live births have resulted from small antral follicles. Furthermore, it is possible to grow primordial follicles to pre-antral stages in slices of ovarian tissue, and support antrum formation in isolated pre-antral follicles. However, we are still a considerable way from growing and maturing pre-antral follicles to pre-ovulatory stages in vitro. The importance of the follicular environment for producing a healthy and developmentally competent oocyte is illustrated by the oocyte's susceptibility to errors during meiosis. This counsels considerable caution in the development of IVM for clinical application.
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