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British Medical Bulletin 56:603-615 (2000)
© 2000 The British Council

research-article

Cryopreservation of gonadal tissue and cells

H M Picton, S S Kim and R G Gosden

Division of Obstetrics and Gynaecology, University of Leeds Leeds, UK

Correspondence to Dr H M Picton, Centre for Reproduction, Growth and Development, Division of obstetrics and Gynaecology, D Floor, Clarendon Wing (LGI), Belmont Grove, Leeds LS2 9NS, UK

Abstract

With the advent of assisted reproductive technology and an improved understanding of cryobiology, strategies have been developed which allow the long-term storage of gametes and embryos. Furthermore, in the light of the growing numbers of young adults and children who have been sterilised by successful cancer treatment, the need to protect their fertile potential has become more urgent. While seman cryopreservation is available for men, the methods for preserving oocytes are unreliable and neither method is suitable for prepubertal children. Research attention is, therefore, focusing on the low temperature banking of immature germ cells with the aim to restore natural fertility if possible or, alternatively, to use culture technology to produce ripe gametes for assisted conception. While there is no universal method for fertility conservation, gonadal tissue banking, in theory, is a practical alternative to gamete storage which can be utilised by both adults and children.


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